Journal: Development (Cambridge, England)

Article Title: Dynamics of thymus organogenesis and colonization in early human development

doi: 10.1242/dev.087320

Figure Lengend Snippet: Expression of markers of cortical and medullary TECs in human thymus development. ( A-K ″) Medullary TEC differentiation. Images show staining with UEA1 (A-D″), anti-CLDN4 (E-G″), anti-CLDN4 and anti-HLA-DR/DP/DQ (MHC II) (H-H″) and anti-AIRE (I-K″). ( L-O ″) Cortical TEC differentiation. Images show staining with CD205 (L-M″) and CDR2 (N-O″). ( P-W ″) Chemokine expression. Images show staining with anti-CCL21 (P-Q″) and anti-CCL25 (R-W″). Developmental stages are as shown. Anti-pan-Cytokeratin (PANK) co-stain reveals the entire thymus primordium. Images are representative of at least two independent analyses. Wk, week. Scale bars: 150 μm in A-B″,D-F″,H-H″,N-W″; 75 μm in C-C″; 47.6 μm in G-G″; 50 μm in I-I″,L-M″; 55 μm in J-K″.

Article Snippet: Primary antibodies used were: anti-cytokeratin (rabbit polyclonal anti-keratin, Dako); anti-CLDN4 (3E2C1, Zymed); anti-human CD45 (clone HI30, BD Pharmingen); anti-keratin 5 (AF138 rabbit IgG, Covance); anti-keratin 8 (CAM 5.2, Becton Dickinson); anti-keratin 14 (AF64, Covance); TE7 (Developmental Studies Hybridoma Bank); UEA1 (biotinylated, Vector Labs); anti-AIRE (D-17, Santa Cruz); anti-CD205 (MG38, Serotec); CDR2 (provided by B. Kyewski, German Cancer Research Center, Heidelberg, Germany); anti-HLA-DR/DP/DQ (clone TU39, BD Pharmingen); anti-EPCAM (Ber-EP4, DakoCytomation); anti-CCL21 (anti-6Ckine, R&D Systems); anti-CCL25/TECK (R&D Systems); mouse anti-CD11b/Mac1 (ICRF44, BD Pharmingen); mouse anti-CD31 (WM59, BD Pharmingen); mouse anti-CD144 PE (16B1, eBiosciences); mouse anti-CD34 FITC (581, BD Pharmingen); mouse anti-CD7 (M-T701, BD Pharmingen).

Techniques: Expressing, Staining

Journal:

Article Title: Mechanisms of binding of cutaneous lymphocyte-associated antigen-positive and ?e?7-positive lymphocytes to oral and skin keratinocytes

doi: 10.1046/j.1365-2567.1999.00855.x

Figure Lengend Snippet: Cell surface expression of ICAM-1 (6.5β5 mAb), E-selectin (BBIG-E4 mAb) and E-cadherin (E4.6 mAb: αeβ7 binding site and HECD-1: homotypic binding site) on oral (H357 and H376) and skin (UP) keratinocyte cell lines and breast cell line (16E6.A5) quantified by cell-based ELISA; n = 6.

Article Snippet: Mouse anti-human αeβ7 (CD103) unconjugated (Serotec, Oxford, UK); rat anti-mouse fluorescein isothiocyanate (FITC) -conjugated immunoglobulin G (IgG; Serotec, Oxford, UK); rat anti-human CLA IgM (HECA-452), and FITC-conjugated negative control rat IgM (Professor L. J. Picker, University of Texas, Dallas, TX); mouse IgG2a FITC-conjugated (Serotec, Oxford, UK); E4.6 mouse anti-E-cadherin specific for the αeβ7 binding epitope 11 (Dr M. Brenner, Boston, MA); HECD-1 mouse anti-E-cadherin, non-blocking (R&D Systems, Abingdon, Oxon, UK); 3S3 mouse anti-β 1 integrin (Serotec, Oxford UK); BerACT8 mouse anti-αeβ7 (Dako, Cambridge, UK) and BBIG-E4 mouse anti-E-selectin (R&D Systems, Oxon, UK).

Techniques: Expressing, Binding Assay, In-Cell ELISA

Journal:

Article Title: Mechanisms of binding of cutaneous lymphocyte-associated antigen-positive and ?e?7-positive lymphocytes to oral and skin keratinocytes

doi: 10.1046/j.1365-2567.1999.00855.x

Figure Lengend Snippet: The percentage adhesion of PHA-activated, IL-12-activated and TGF-β-activated PBL to the E-cadherin-positive H357 oral keratinocyte cell line. Lymphocytes were incubated with or without antibodies against αeβ7 (BerACT8), LFA-1 (TS1/18), β1 integrin (3S3), αeβ7 binding site on E-cadherin (E4.6) and the homotypic binding site on E-cadherin (HECD-1). Essentially similar results were found with the skin cell line UP – results not shown. For each experiment n = 6 or above.

Article Snippet: Mouse anti-human αeβ7 (CD103) unconjugated (Serotec, Oxford, UK); rat anti-mouse fluorescein isothiocyanate (FITC) -conjugated immunoglobulin G (IgG; Serotec, Oxford, UK); rat anti-human CLA IgM (HECA-452), and FITC-conjugated negative control rat IgM (Professor L. J. Picker, University of Texas, Dallas, TX); mouse IgG2a FITC-conjugated (Serotec, Oxford, UK); E4.6 mouse anti-E-cadherin specific for the αeβ7 binding epitope 11 (Dr M. Brenner, Boston, MA); HECD-1 mouse anti-E-cadherin, non-blocking (R&D Systems, Abingdon, Oxon, UK); 3S3 mouse anti-β 1 integrin (Serotec, Oxford UK); BerACT8 mouse anti-αeβ7 (Dako, Cambridge, UK) and BBIG-E4 mouse anti-E-selectin (R&D Systems, Oxon, UK).

Techniques: Incubation, Binding Assay

Journal:

Article Title: Mechanisms of binding of cutaneous lymphocyte-associated antigen-positive and ?e?7-positive lymphocytes to oral and skin keratinocytes

doi: 10.1046/j.1365-2567.1999.00855.x

Figure Lengend Snippet: The percentage adhesion of PHA-activated and TGF-β-activated PBL to the breast cell line 16E6.A5. Lymphocytes were incubated either with or without antibodies against the homotypic binding site on E-cadherin (HECD-1), the αeβ7 binding site on E-cadherin (E4.6), LFA-1 (TS1/18) and αeβ7 (BerACT8). For each experiment n = 6 or above.

Article Snippet: Mouse anti-human αeβ7 (CD103) unconjugated (Serotec, Oxford, UK); rat anti-mouse fluorescein isothiocyanate (FITC) -conjugated immunoglobulin G (IgG; Serotec, Oxford, UK); rat anti-human CLA IgM (HECA-452), and FITC-conjugated negative control rat IgM (Professor L. J. Picker, University of Texas, Dallas, TX); mouse IgG2a FITC-conjugated (Serotec, Oxford, UK); E4.6 mouse anti-E-cadherin specific for the αeβ7 binding epitope 11 (Dr M. Brenner, Boston, MA); HECD-1 mouse anti-E-cadherin, non-blocking (R&D Systems, Abingdon, Oxon, UK); 3S3 mouse anti-β 1 integrin (Serotec, Oxford UK); BerACT8 mouse anti-αeβ7 (Dako, Cambridge, UK) and BBIG-E4 mouse anti-E-selectin (R&D Systems, Oxon, UK).

Techniques: Incubation, Binding Assay

Journal:

Article Title: Mechanisms of binding of cutaneous lymphocyte-associated antigen-positive and ?e?7-positive lymphocytes to oral and skin keratinocytes

doi: 10.1046/j.1365-2567.1999.00855.x

Figure Lengend Snippet: Adhesion of PHA-activated, IL-12-activated and TGF-β-activated PBL to the E-cadherin-negative oral keratinocytes cell line H376. Lymphocytes were incubated either with or without antibodies to αeβ7 (BerACT8), LFA-1 (TS1/18), β1 integrin (3S3), αeβ7 binding site on E-cadherin (E4.6) and the homotypic binding site on E-cadherin (HECD-1). For each experiment n = 6 or above.

Article Snippet: Mouse anti-human αeβ7 (CD103) unconjugated (Serotec, Oxford, UK); rat anti-mouse fluorescein isothiocyanate (FITC) -conjugated immunoglobulin G (IgG; Serotec, Oxford, UK); rat anti-human CLA IgM (HECA-452), and FITC-conjugated negative control rat IgM (Professor L. J. Picker, University of Texas, Dallas, TX); mouse IgG2a FITC-conjugated (Serotec, Oxford, UK); E4.6 mouse anti-E-cadherin specific for the αeβ7 binding epitope 11 (Dr M. Brenner, Boston, MA); HECD-1 mouse anti-E-cadherin, non-blocking (R&D Systems, Abingdon, Oxon, UK); 3S3 mouse anti-β 1 integrin (Serotec, Oxford UK); BerACT8 mouse anti-αeβ7 (Dako, Cambridge, UK) and BBIG-E4 mouse anti-E-selectin (R&D Systems, Oxon, UK).

Techniques: Incubation, Binding Assay

Journal: Heliyon

Article Title: Expression of tolerogenic dendritic cells in the small intestinal tissue of patients with celiac disease

doi: 10.1016/j.heliyon.2022.e12273

Figure Lengend Snippet: Forward and reverse sequences of IDO, CD11c, CD103, CD207, and Β 2 m primers.

Article Snippet: For immunohistochemical analysis the following ready-to-use antibodies (Master Diagnostica, Spain) including monoclonal rabbit anti-human CD11c (Clone EP157monoclonal rabbit anti-human CD103 (Clone EP206) and monoclonal rabbit anti-human Langerin CD207 (Clone EP15863) were used.

Techniques: Sequencing

Journal: Heliyon

Article Title: Expression of tolerogenic dendritic cells in the small intestinal tissue of patients with celiac disease

doi: 10.1016/j.heliyon.2022.e12273

Figure Lengend Snippet: Genes expression analysis in CeD patients compared to control group. IDO mRNA expression (A); CD11c mRNA expression (B); CD103 mRNA expression (C); CD207 mRNA expression (D). Statistical analysis: Kruskall–Wallis anova and Man Whitney u. ∗, ∗∗, ∗∗∗P value is significant IDO: Indoleamine 2,3-dioxygenase.

Article Snippet: For immunohistochemical analysis the following ready-to-use antibodies (Master Diagnostica, Spain) including monoclonal rabbit anti-human CD11c (Clone EP157monoclonal rabbit anti-human CD103 (Clone EP206) and monoclonal rabbit anti-human Langerin CD207 (Clone EP15863) were used.

Techniques: Expressing, Control

Journal: Heliyon

Article Title: Expression of tolerogenic dendritic cells in the small intestinal tissue of patients with celiac disease

doi: 10.1016/j.heliyon.2022.e12273

Figure Lengend Snippet: Analysis of (A) CD11c, (B) CD103, and (C) CD207 protein expression in the small intestinal tissue by immunohistochemistry. Positive staining for CD11c in the human tonsil tissue, CD103 in the T lymphocytes of the small intestine mucosa, CD207 in the human skin tissue. Indirect method. Original magnification, 40× objective; 10× eyepiece.

Article Snippet: For immunohistochemical analysis the following ready-to-use antibodies (Master Diagnostica, Spain) including monoclonal rabbit anti-human CD11c (Clone EP157monoclonal rabbit anti-human CD103 (Clone EP206) and monoclonal rabbit anti-human Langerin CD207 (Clone EP15863) were used.

Techniques: Expressing, Immunohistochemistry, Staining

Journal: Heliyon

Article Title: Expression of tolerogenic dendritic cells in the small intestinal tissue of patients with celiac disease

doi: 10.1016/j.heliyon.2022.e12273

Figure Lengend Snippet: The mean values of CD11c expression in the studied groups ((A) LP expression, (B) EP expression, (C) total expression). Columns represent the mean values of positively stained cells per microscopic field in patients with CeD vs healthy controls. Statistical analysis: Kruskall–Wallis anova and Man Whitney u. LP: lamina propria; EP: Epithelial layer.

Article Snippet: For immunohistochemical analysis the following ready-to-use antibodies (Master Diagnostica, Spain) including monoclonal rabbit anti-human CD11c (Clone EP157monoclonal rabbit anti-human CD103 (Clone EP206) and monoclonal rabbit anti-human Langerin CD207 (Clone EP15863) were used.

Techniques: Expressing, Staining

Journal: Heliyon

Article Title: Expression of tolerogenic dendritic cells in the small intestinal tissue of patients with celiac disease

doi: 10.1016/j.heliyon.2022.e12273

Figure Lengend Snippet: Correlations between mRNA expressions of (A) IDO and CD11c, (B) IDO and CD103, (C) IDO and CD207, (D) CD11c and CD103, (E) CD103 and CD207, (F) CD11c and CD207. The letter “r” represents correlation coefficient between two variables. P value <0.05 was considered as statistically significant. IDO: Indoleamine 2, 3-dioxygenase.

Article Snippet: For immunohistochemical analysis the following ready-to-use antibodies (Master Diagnostica, Spain) including monoclonal rabbit anti-human CD11c (Clone EP157monoclonal rabbit anti-human CD103 (Clone EP206) and monoclonal rabbit anti-human Langerin CD207 (Clone EP15863) were used.

Techniques:

Journal: Heliyon

Article Title: Expression of tolerogenic dendritic cells in the small intestinal tissue of patients with celiac disease

doi: 10.1016/j.heliyon.2022.e12273

Figure Lengend Snippet: Correlations between proteins expressions of (A) CD11c and CD103, (B) CD11c and CD207, and (C) CD103 and CD207 were examined by Pearson's correlation test. The letter “r” represents correlation coefficient between two variables. P value <0.05 was considered as statistically significant.

Article Snippet: For immunohistochemical analysis the following ready-to-use antibodies (Master Diagnostica, Spain) including monoclonal rabbit anti-human CD11c (Clone EP157monoclonal rabbit anti-human CD103 (Clone EP206) and monoclonal rabbit anti-human Langerin CD207 (Clone EP15863) were used.

Techniques:

Journal: Heliyon

Article Title: Expression of tolerogenic dendritic cells in the small intestinal tissue of patients with celiac disease

doi: 10.1016/j.heliyon.2022.e12273

Figure Lengend Snippet: Areas under the curve values and the cut-off, sensitivity, and specificity values for genes and proteins dendritic cells by qRT-PCR and Immunohistochemistry on paraffin-embedded specimens.

Article Snippet: For immunohistochemical analysis the following ready-to-use antibodies (Master Diagnostica, Spain) including monoclonal rabbit anti-human CD11c (Clone EP157monoclonal rabbit anti-human CD103 (Clone EP206) and monoclonal rabbit anti-human Langerin CD207 (Clone EP15863) were used.

Techniques: Immunohistochemistry